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| CD45 Flow Cytometry Test |
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| Detects CD 45 markers to diagnose leukemia or lymphoma, causing fatigue or swollen lymph nodes | ||
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Synonym | CD 45 Flow Test |
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Test Code | CHEM250030 |
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Test Type | Hematology |
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Pre-Test Condition | No special |
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Report Availability | 1–2 D(s) |
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# Test(s) | 1 |
| Test details | Sample Report |
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| CD45 Flow Cytometry Test |
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| Synonym | CD 45 Flow Test | |
| Test Code | CHEM250030 | |
 | Test Category | ||
| Pre-Test Condition | No special | |
 | Medical History | Share & see Updates | |
| Report Availability | 1–2 D(s) | |
 | Specimen/Sample | Refer Updates | |
 | Stability @21-26 deg. C | 24 H(s) | |
 | Stability @ 2-8 deg. C | 48 H(s) | |
 | Stability @ Frozen | Not frozen | |
| # Test(s) | 1 | |
 | Processing Method | Flow Cytometry | |
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Overview: CD45 Flow Cytometry Test
Introduction: The CD45 Flow Cytometry Test detects CD45 markers to diagnose leukemia or lymphoma, causing fatigue or swollen lymph nodes. Aligned with 2023 ASH guidelines, it uses flow cytometry for high specificity, aiding in cancer screening. This test is critical for guiding diagnosis, treatment, and improving outcomes in hematology for patients with suspected hematologic malignancies. Other Names: CD45 Flow Assay, Leukocyte Common Antigen Test. FDA Status: Laboratory-developed test (LDT), meeting hematology standards for diagnostic accuracy. Historical Milestone: CD45 testing began in the 1980s with leukemia research. Flow cytometry emerged in the 1990s, and standardized panels improved accuracy by the 2000s. Purpose: Diagnoses leukemia or lymphoma, guides treatment, and monitors CD45 markers in patients with fatigue or swollen lymph nodes. Test Parameters: 1. CD45 Pretest Condition: No fasting required. Collect whole blood or bone marrow at any time. Report symptoms like fatigue or swollen lymph nodes, and list medications. Specimen: 2-5 mL whole blood (EDTA) or 2-5 mL bone marrow (EDTA). Transport in a biohazard bag within 24 hours. Sample Stability at Room Temperature: 24 hours Sample Stability at Refrigeration: 48 hours Sample Stability at Frozen: Not frozen Medical History: Document fatigue, swollen lymph nodes, or history of leukemia/lymphoma. Include current medications, especially chemotherapeutics. Consent: Written consent required, detailing the tests purpose, diagnostic implications, and potential need for cancer therapy. Procedural Considerations: Uses flow cytometry to detect CD45 markers. Results are available in 1-2 days, enabling rapid clinical decisions. Factors Affecting Result Accuracy: Poor sample quality or low cell yield can affect results. Non-malignant conditions may express CD45. Clinical Significance: Positive CD45 expression suggests leukemia or lymphoma, prompting treatment. Negative results may require additional marker testing. Specialist Consultation: Consult a hematologist or oncologist for result interpretation. Additional Supporting Tests: Bone marrow biopsy, other flow cytometry markers, or PET scan to confirm leukemia/lymphoma. Test Limitations: Non-specific expression may occur in non-malignant conditions. Results require clinical correlation. References: ASH Leukemia Guidelines, 2023; Blood, Arber DA, 2022. |