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| Sickle-Cell Screening Test |
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| Screens for sickle cell trait to diagnose anemia, causing pain or fatigue | ||
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Synonym | Sickling Test |
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Test Code | CHEM250074 |
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Test Type | Hematology |
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Pre-Test Condition | No special |
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Report Availability | 1–2 D(s) |
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# Test(s) | 1 |
| Test details | Sample Report |
|---|---|
| Sickle-Cell Screening Test |
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| Synonym | Sickling Test | |
| Test Code | CHEM250074 | |
 | Test Category | ||
| Pre-Test Condition | No special | |
 | Medical History | Share & see Updates | |
| Report Availability | 1–2 D(s) | |
 | Specimen/Sample | Refer Updates | |
 | Stability @21-26 deg. C | 24 H(s) | |
 | Stability @ 2-8 deg. C | 48 H(s) | |
 | Stability @ Frozen | Not frozen | |
| # Test(s) | 1 | |
 | Processing Method | Hematology | |
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Overview: Sickle-Cell Screening Test
Introduction: The Sickle-Cell Screening Test screens for sickle cell trait to diagnose anemia, causing pain or fatigue. Following 2023 ASH guidelines, it uses hematological methods for high specificity, supporting blood disorder screening. This test is critical for guiding diagnosis, treatment planning, and improving outcomes in hematology for patients with suspected sickle cell disease or trait. Other Names: Sickling Test, Sickle Cell Trait Assay. FDA Status: Laboratory-developed test (LDT), meeting hematology standards for diagnostic accuracy. Historical Milestone: Sickle cell screening began in the 1940s with hemoglobin research. Methods improved in the 2000s, enhancing diagnostic precision. Purpose: Screens for sickle cell trait or disease, guides treatment, and evaluates patients with pain or fatigue. Test Parameters: 1. Sickling Presence Pretest Condition: No fasting required. Collect whole blood. Report history of pain, fatigue, or sickle cell symptoms. Specimen: Whole Blood (EDTA, 2-5 mL). Transport in a biohazard container. Sample Stability at Room Temperature: 24 hours Sample Stability at Refrigeration: 48 hours Sample Stability at Frozen: Not frozen Medical History: Document pain, fatigue, or family history of sickle cell disease/trait. Include current medications, especially hydroxyurea. Consent: Written consent required, detailing the tests purpose, sickle cell implications, and risks of sample collection. Procedural Considerations: Uses sodium metabisulfite or solubility tests to detect sickling. Results are available in 1-2 days, supporting clinical decisions. Performed in laboratories, often for sickle cell screening. Factors Affecting Result Accuracy: Improper sample storage or hemolysis can affect results. Recent transfusions may alter results. Clinical Significance: Positive sickling confirms sickle cell trait or disease, guiding genetic counseling or therapy. Negative results may require mutation testing. Specialist Consultation: Consult a hematologist for result interpretation and treatment planning. Additional Supporting Tests: Hemoglobin electrophoresis, HBB mutation testing, or CBC to confirm sickle cell diagnosis. Test Limitations: Non-specific for trait vs. disease; clinical correlation is needed. Sample quality affects sensitivity. References: ASH Sickle Cell Guidelines, 2023; Blood, Rees DC, 2022. |